Fast Protein Liquid Chromatography (FPLC) Systems (2x)

Fast protein liquid chromatography (FPLC) is often used to analyze or purify mixtures of proteins. The ÄKTApurifier FPLC system is a fully automated liquid chromatography system designed for protein purification, characterization, and collection, wherein proteins of various sizes can be readily purified using different kinds of columns. In addition, this system is also ideal for lipoprotein-based, liposome-based nanoparticle purification.

There are two ÄKTApurifier FPLC systems in the NanoMedFab. Both systems are equipped with UV, pH, and conductivity  detectors. The purified samples are collected through an automated fraction collector. In addition, one system is equipped with an autosampler for automated sample injections. The NanoMedFab also provides several types of prep grade columns for the FPLC systems.

Specifications and features

• UV spectral range: 190 to 700 nm
• Multiple wavelength detection: up to 3
• Conductivity range: 1 µS/cm to 999.9 mS/cm
• pH range: 0 to 14 pH units
• Collection tube format: 12 mm (i.e. 15 mL falcon tube), 18 mm, 30 mm, 96-well microplates.
• Flow rate: 0.001 to 10 mL/min
• Injection volume: maximum 5 mL
• Columns available in NanoMedFab: HiLoad XK 16/70 Superose 6 pg and HiLoad 16/600 Superdex 200 pg